Emerging healthcare needs, including global healthcare, personalized medicine, and point-of-care applications are demanding breakthrough advancements in diagnostic and bioanalytical tools. Towards this goal, the Altug lab at EPFL is developing next-generation nanophotonic lab-on-a-chip systems offering high performance in accuracy, response time, integration, throughput, and affordability while reducing complexity, cost and device footprint. Its researchers are building optical biosensors, spectroscopy, bioimaging and microarray technologies to sensitively detect and analyze biological samples, including disease biomarkers, misfolded protein aggregates, nucleic acids, drugs, and living cells. To achieve these objectives, they uniquely combine nanophotonics with advanced nanofabrication, microfluidics, surface chemistry, and data science techniques. In particular, they engineer optical metasurfaces exploiting plasmonics and dielectric resonators to increase the interaction of light with nanometric-sized biomolecules fundamentally, and depending on the detection principle, their operation wavelength is controlled within a broad spectrum ranging from visible to mid-infrared. The laboratory utilizes low-cost and wafer-scale nanofabrication methods for manufacturing of nanophotonic metasurfaces. The researchers integrate metasurfaces with microfluidic systems for efficient analyte handing. They leverage data science tools to achieve high sensor performance. In this talk, Prof. Altug will present some of her lab's recent works on surface enhanced mid-infrared spectroscopy such as an AI-aided Mid-IR optofluidic biosensor capable of differentiating misfolded disease proteins and high-Q gradient mid-IR metasurfaces for ultra-broadband operation, describe nanophotonic single-cell and organoid microarrays capable of high-throughput monitoring of extracellular secretion for screening applications and introduce biosensors that can enable continuous monitoring.